Safe, efficient Stem cell type: Induced pluripotent stem cells Generates integration free, virus-free human iPS cell Single transfection step of self-replicating synthetic RNA for Oct4, Klf4, Sox2, Glis1 Integration free, footprint free iPS cells No risk of genomic integration Safe, virus free, synthetic polycistronic RNA replicon (all four reprogramming factors in 1 RNA str) Only 1 day transfection required RNA replicon is able to self-replicate, elliminating need for laborious daily transfection of multiple individual mRNAs over 14 day period Efficient rapid reprogramming No screening required to ensure viral remnants are not present Controlled elimination of synthetic RNA replicon by removal of B18R protein Validated for reprogramming in feeder-based feeder-free culture conditions