Invitrogen™ X-Rhod-1, AM, cell permeant - Special Packaging

Description

• Uses in many calcium signaling investigations, including measuring Ca 2+ in cells and tissues Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells Fluorescence signal from these cells is generally measured using fluorescence microscopy Exhibit fluorescence increase upon binding Ca 2+ with little shift in wavelength In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn 2+ , Zn 2+ , Pb 2+ ) with substantially higher affinity than Ca 2+ Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator Label (Ex/Em of Ca 2+ –bound form): X-rhod-1 (580/602nm) Fluorescence intensity increase upon binding Ca 2+ : >100 fold K d for Ca 2+ in the absence of Mg 2+ , in buffer: approximately 700nM Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling Order Info Shipping Condition: Room temperature