Invitrogen™ Olympus™ 20X Objective, fluorite, 0.45NA/6.6–7.8WD, correction collar (0–2.0 mm)

Description

• For the sharpest image simply set the collar to the thickness of the vessel/coverslip you are using
• If the thickness is unknown, turn the collar in either direction while monitoring the sample focus
• All Olympus objectives are compatible with EVOS cell imaging systems and offer outstanding optical performance from visible to near-infrared light
• The extensive selection of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications
• Additional characteristics of this Olympus objective: Magnification : 20X Numerical Aperture : 0.45 Working Distance : 6.6–7.8 mm Superb image quality Image quality Microscope objectives may be the most important components of a microscope because they are responsible for primary image formation
• High image quality is crucial to experimental success and often a requirement for publication; Olympus objectives afford that quality across the visible spectrum to near infrared light
• This performance results from years of lens manufacture experience, and the broad selection of objectives means that you can choose the optimal objective for your imaging requirements
• Objective classes Achromat objectives are perfect for general applications, with standard correction of color and focus
• Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging
• The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives
• Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging
• Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated
• They are recommended for the most demanding applications, particularly at magnifications of 60x and above
• Apochromatic objectives are the best choice for the capture of color images in white light
• Brightfield contrast versus phase contrast objectives Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light
• A higher density area in a sample will absorb more light, thus increasing contrast in those areas
• Phase contrast objectives are most useful for hard to see, translucent specimens
• This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast)
• Long working distance versus coverslip-corrected objectives Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm
• This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc
• Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm)
• These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.