Invitrogen™ EVOS™ Light Cube, RFP 2.0

Description

• Optimized spectral fidelity across channels significantly improves data quality by minimizing undesired bleed-through
• Like the original design, they combine condensing optics and hard-coated filters into one remarkable light engine that delivers digitally controlled fluorophore excitation
• These LED light cubes are backwards compatible with older EVOS and Countess systems and can be used with first-generation light cubes
• Like the original light cubes, these LED cubes are user-changeable and automatically recognized by the instrument
• The EVOS Light Cube, RFP 2.0, is ideal for use with Alexa Fluor 555, Cy3, MitoTracker Orange CMTMRos, pHrodo, RFP, Rhod-2, and other fluorophores with excitation and emission maxima near 542 and 593 nm
• The EVOS Light Cube, RFP 2.0, offers you these important advantages: Uniform illumination across the entire field of view for superb image quality Optimized spectral compatibility to maximize true signal detection, while minimizing bleed-through for the best possible data quality A short light path that affords superior detection of fluorescent signals Stable illumination intensity over 50,000 h helps experimental reproducibility Efficient light engines The second-generation light cube design dramatically improves on the original
• A larger aperture increases illumination uniformity, which affords superb tile-stitched image quality and consistency
• In addition, new LED light and filter combinations maximize the signal-to-background ratio so critical for dim samples, improving data quality irrespective of signal strength for both EVOS and Countess systems
• By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized
• High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals
• Hard-coated filter sets for higher transmission efficiencies Hard-coated filters are more expensive but have sharper edges and significantly higher transmission efficiencies that typically result in >25% higher light transmission compared to soft-coated filters
• LED bulbs and filters optimized to maximize the signal from target fluorophores, while minimizing that from other dyes (i.e., bleed-through), will help you obtain brighter fluorescence, higher transmission efficiencies and ability to detect faint fluorescence signals, and better signal-to-noise ratios
• These factors raise both image and data qualities
• Continuous light intensity Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations
• EVOS cubes retain continuous light cube intensity that affords consistent illumination from day-to-day and experiment-to-experiment and help you to directly compare quantitative results from images acquired on different days
• Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam
• These digitally controlled light sources make it easy to adjust light intensity, exposure time, and camera gain that can reduce phototoxicity and photobleaching.