Invitrogen™ eBioscience™ Mouse Th17 Cytokine Staining Panel

Description

• This mouse Th17 cytokine staining panel includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage: IL-17A, IL-17F, IL-21 and IL-22
• An anti-CD4 antibody that can be used after fixation and permeabilization, as well as IC Fixation and Permeabilization Buffers, is also included
• CD4+ T helper cells are critical mediators of the cellular immune response
• For many years, due to cytokine expression patterns, it was thought that CD4+ T helper cells existed as a dichotomy of lineages named Th1 and Th2
• However, further investigation revealed that the T helper cell population was not limited to these two subsets
• Although it had long been appreciated that IL-17 (also known as IL-17A) production by T cells was required for protection against some pathogens, studies demonstrated that this cytokine was produced by a unique subset of T helper cells
• Subsequent reports definitively showed that T cells could differentiate into IL-17-producing cells in vitro and in vivo independent of Th1 or Th2 development, thereby establishing Th17 cells as a unique T helper cell lineage
• In addition to IL-17A expression, Th17 cells have been reported to express IL-17F (and heterodimers of Il-17 and Il-17F), IL-21 and IL-22
• Functionally, Th17 cells play a role in host defense against extracellular pathogens by mediating the recruitment of neutrophils and macrophages to infected tissues
• Moreover, it is becoming evident that aberrant regulation of Th17 cells may play a significant role in the pathogenesis of multiple inflammatory and autoimmune disorders
• Reactivity/Species: Mouse Reported Application: Intracellular Staining followed by Flow Cytometric Analysis