Molecular Probes™ AbC™ Anti-Mouse Bead Kit

Description

• The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry
• Species reactivity —for use with all isotypes of mouse antibody conjugates Maximum laser compatibility —for use with all lasers Accurate compensation is crucial for effective multicolor analysis in flow cytometry
• Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings
• The AbC™ Anti-Mouse Bead Kit was developed to address this issue
• How it works Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads)
• After mixing with a fluorophore-conjugated mouse antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment
• Species reactivity The AbC™ Anti-Mouse Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM) immunoglobulins
• Maximum laser compatibility All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.