Molecular Probes™ AbC™ Anti-Rat/Hamster Bead Kit

Description

• The AbC™ Anti-Rat/Hamster Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated to rat or hamster antibodies with any laser in flow cytometry
• Species reactivity —for use with all isotypes of rat and hamster antibody conjugates Maximum laser compatibility —for use with all lasers Accurate compensation is crucial for effective multicolor analysis in flow cytometry
• Often, however, cellular-baseThe AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry
• Species reactivity —for use with all isotypes of mouse antibody conjugates Maximum laser compatibility —for use with all lasers Accurate compensation is crucial for effective multicolor analysis in flow cytometry
• Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings
• The AbC™ Anti-Mouse Bead Kit was developed to address this issue
• How it works Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads)
• After mixing with a fluorophore-conjugated mouse antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment
• Species reactivity The AbC™ Anti-Mouse Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM) immunoglobulins
• Maximum laser compatibility All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.d compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings
• The AbC™ Anti-Rat/Hamster Bead Kit was developed to address this issue
• How it works Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads)
• After mixing with a fluorophore-conjugated rat or hamster antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment
• Species reactivity The AbC™ Anti-Rat/Hamster Bead Kit is ideal for compensation with all isotypes of rat (IgG1, IgG2a, IgG2b, IgG2c, IgM) and hamster (Armenian IgG and Syrian IgG) immunoglobulins
• Maximum laser compatibility All AbC™ Compensation Bead Kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.