A43676
Applied Biosystems™ PureQuant™ Th17 Assay
Manufacturer: Applied Biosystems™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A43676-Each-of-1 | In Stock | ₹ 3,45,662.40 |
A43676 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,45,662.40
GST (18%): ₹ 62,219.232
Total Price: ₹ 4,07,881.632
Quantity
28 reactions
Detection Method
Primer-Probe Detection
Shipping Condition
Approved for shipment at Room Temperature or on Wet or Dry Ice
Content And Storage
Dynabeads SILANE Genomic DNA Kit, store at 2°C to 8°CC1 x 2 mL PureQuant qPCR Master Mix, store at 2°C to 8°CC4 x ABSTHFA, store at room temperaturePureQuant Th17 Module, store at -15 to -25°C (CpG & TpG TaqMan primers and probes, Reference DNA, Lambda DNA, Standard DNA)PureQuant Hot Start PCRPureQuant Pre-amplification primer
For Use With (Application)
Cells, DNA
Related Products
Description
- Assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier
- The assays include standards and controls and each 96-well plate can accommodate up to seven test samples
- Results are provided as a percentage of target cell type out of total input cells using a free analysis tool
- PureQuant™ Th17 Assay utilizes a specific demethylated site in the pro-inflammatory cytokine interleukin 17A (IL17)
- This cytokine is exclusively produced by T helper 17 cells
- Benefits include: Proven performance ―core technology has been used for identification and quantification of immune cell types for over a decade Easily standardized ―qPCR method enables consistent results across multiple users, instruments, and sites Facilitates regulatory filings ―regulatory support file provided upon request; established performance specifications following ICHQ2(R1) guidelines Scheduling flexibility ―assay can be performed using fresh or frozen cells/gDNA Assays include all reagents, standards, and controls required to run the assay
- Specific components include: Calibrator: Plasmid-based calibrator harbors the genomic target regions in equimolar ratios and includes GAPDH
- Subjected to bisulfite conversion followed by copy number estimation via qPCR standard curve
- Allows estimation of assay-specific bisulfite efficiency factor: the ratio between TpG/GAPDH copy numbers measured from the same plasmid
- Reference: checks assay performance
- Consists of genomic DNA extracted from human blood of pooled healthy donors
- The reference values represent the expected range of the target cell type
- For example, CD8+ cells are generally more abundant than Treg or Th17 cells
- Standards: determine the unknown copy number
- Consist of six dilutions spanning 4-log linear dynamic range (30-31250 copies)
- Absolute copy numbers can reliably be determined using the slope and intercept of the respective standard curves
- This product is manufactured at a site with an ISO9001-certified quality management system.
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Quantity: 28 reactions
Detection Method: Primer-Probe Detection
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Content And Storage: Dynabeads SILANE Genomic DNA Kit, store at 2°C to 8°CC1 x 2 mL PureQuant qPCR Master Mix, store at 2°C to 8°CC4 x ABSTHFA, store at room temperaturePureQuant Th17 Module, store at -15 to -25°C (CpG & TpG TaqMan primers and probes, Reference DNA, Lambda DNA, Standard DNA)PureQuant Hot Start PCRPureQuant Pre-amplification primer
For Use With (Application): Cells, DNA
Quantity:
28 reactions
Detection Method:
Primer-Probe Detection
Shipping Condition:
Approved for shipment at Room Temperature or on Wet or Dry Ice
Content And Storage:
Dynabeads SILANE Genomic DNA Kit, store at 2°C to 8°CC1 x 2 mL PureQuant qPCR Master Mix, store at 2°C to 8°CC4 x ABSTHFA, store at room temperaturePureQuant Th17 Module, store at -15 to -25°C (CpG & TpG TaqMan primers and probes, Reference DNA, Lambda DNA, Standard DNA)PureQuant Hot Start PCRPureQuant Pre-amplification primer
For Use With (Application):
Cells, DNA
Quantity: 1 kit
Detection Method: Primer-probe
Shipping Condition: Dry Ice
Content And Storage: Each kit contains enough PCR Master Mix to perform 50 PCR amplifications, 5 negative control assays, and 5 positive control assays. One tube of positive control E. coli DNA, and one of water (for use as a negative control) are also included.Store at -15°C to -25°C upon reciept. After first use, store at 2°C to 8°C in a PCR Clean Room.
For Use With (Application): Standard PCR
Quantity:
1 kit
Detection Method:
Primer-probe
Shipping Condition:
Dry Ice
Content And Storage:
Each kit contains enough PCR Master Mix to perform 50 PCR amplifications, 5 negative control assays, and 5 positive control assays. One tube of positive control E. coli DNA, and one of water (for use as a negative control) are also included.Store at -15°C to -25°C upon reciept. After first use, store at 2°C to 8°C in a PCR Clean Room.
For Use With (Application):
Standard PCR
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