Invitrogen™ CellBlox™ Blocking Buffer

Description

• Formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells that can result in higher background labeling CellBlox™ Blocking Buffer is a non-antibody, non-protein–based blocking solution, and should be used every time a NovaFluor dye is used for labeling any cell type to minimize background labeling Recommended for use with cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling Use of CellBlox Blocking Buffer requires minimal change to most flow cytometry staining protocols Add 5 μL of buffer directly to a cell suspension containing 10 3 to 10 8 cells prior to the addition of an antibody, with 100 μL as a final staining volume Buffer may be added to an antibody cocktail mixture prior to labeling cells, by adding 5 μL of buffer for every stained sample to be labeled with the antibody cocktail mixture, with 100 μL as a final staining volume Always use CellBlox Blocking Buffer with NovaFluor dyes when labeling cells for best background reduction Compatible with all fluorophores and with Invitrogen™ LIVE/DEAD™ Fixable Dead Cell Stains Can be used with any fluorophore-antibody conjugate as a high-performance monocyte and macrophage blocking solution CellBlox Blocking Buffer is compatible with other blocking reagents such as Fc Block, blocking proteins, Brilliant Stain Buffer, and Super Bright Complete Staining Buffer Buffer is not required when labeling antibody-capture beads