BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer

Description

• Some cyanine-type dyes (PE-Cy5, PerCP-Cy5.5, PE-Cy7, APC-Cy7, APC-H7, and others) and non-cyanine tandem dyes (eg, PE-CF594) are known to bind to human and mouse monocytes, macrophages, and other leukocyte subsets, though the exact mechanism is not clear
• BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces this nonspecific background staining, while not impacting specific surface staining or cell viability
• This buffer is added directly to leukocytes or added to fluorescent reagent cocktails prior to staining leukocytes
• This reagent may be complemented by others including i) BD Horizon™ Brilliant Stain Buffer, which mitigates dye-dye interactions amongst BD Horizon™ Brilliant fluorochromes, and ii) BD Fc Block™ reagents
• The latter can reduce potential nonspecific staining caused by fluorescent antibodies that bind to Fc receptors expressed by human and mouse cells
• For optimal and reproducible surface staining results, BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer should be used with cyanine-like and other tandem dye-conjugated reagents that can potentially stain cells nonspecifically
• Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.