Invitrogen™ CellBlox™ Plus Blocking Buffer

Description

• Invitrogen CellBlox Plus Blocking Buffer is formulated to block nonspecific binding of NovaFluor labels with cells
• These nonspecific interactions can result in higher background labeling
• CellBlox Plus Blocking Buffer is a non-antibody blocking solution and should be used whenever a NovaFluor dye is used for labeling of any cell type
• CellBlox Plus Blocking Buffer has enhanced blocking capabilities compared to our original CellBlox Blocking Buffer (Figure 1)
• CellBlox Plus Blocking Buffer is also recommended for use with cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling (Figure 2)
• Features of CellBlox Plus Blocking Buffer include: • Compatible with all fluorophores and with LIVE/DEAD Fixable dead cell stains • Can be used with any fluorophore-antibody conjugate as high-performance monocyte and macrophage blocking solution • Compatible with other blocking reagents, such as Fc Block, blocking proteins, Brilliant Stain Buffer, and Super Bright Complete Staining Buffer • Not required when labeling antibody-capture beads, such as UltraComp eBeads Plus Compensation Beads Use of CellBlox Plus Blocking Buffer requires minimal change to most flow cytometry staining protocols
• Simply add CellBlox Plus Blocking Buffer to an antibody cocktail mixture prior to labeling cells
• Specifically, add 5 μL CellBlox Plus Blocking Buffer for every stained sample to be labeled with the antibody cocktail mixture, with 100 μL as a final staining volume.