Invitrogen™ Dynabeads™ His-Tag Isolation and Pulldown

Description

• Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets
• Their small but uniform bead size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule
• They enable single-step isolation of tagged molecules in manual or automated workflows that helps ensure good recovery of the target
• Dynabeads His-Tag Isolation and Pulldown beads can also be a great starting point for other advanced experimental approaches where solid magnetic support is needed
• Benefits of Dynabeads His-Tag and Pulldown beads include: • Performance —high sensitivity and reproducibility securing consistent results • Efficiency —fast binding kinetics and high binding capacity • Flexibility —flexible protocols with gentle and efficient liquid-phase reaction kinetics • Automation-ready —automation protocols available with the KingFisher Purification instruments Good signal-to-noise for numerous applications Dynabeads His-Tag Isolation and Pulldown beads are an ideal tool for isolation of tagged recombinant proteins
• They are also suited for other applications with high signal-to-noise requirements
• Using the principles of immobilized metal affinity binding together with the inert surface of Dynabeads magnetic beads helps ensure a clean capture with extremely low carryover of unwanted proteins
• Since the bead surface is loaded with cobalt ions, it is also possible to make a stable and lasting bond with the target molecule through oxidation if the downstream applications require it
• The beads are added to the solution containing the target and mixed for 5-10 minutes
• After washing, the target is eluted off and ready for downstream applications
• More than just a pulldown In addition to being a great tool for protein pulldown and isolation, the Dynabeads His-Tag Isolation and Pulldown beads can serve as a platform for target immobilization
• The bead carries cobalt Co2+ ions, so it is possible to cement the bond between the bead and the target just by using mildly oxidative conditions, such as 10 mM hydrogen peroxide for 30–60 minutes
• This oxidizes cobalt to the Co3+ state, making the connection between the bead and the target permanent
• It will not elute in the commonly used elution buffers for IMAC
• This can later be used for applications requiring a strongly bound target, without the risk of reduced target yield
• Automation-ready for use with KingFisher purification systems The 1-μm magnetic beads are ideal for high-throughput enrichment
• This process can be automated using any of our KingFisher sample purification systems or liquid handlers
• Commercial supply Our manufacturing sites are ISO 13485-certified
• Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply
• If you are in the process of customizing Dynabeads His-Tag Isolation and Pulldown beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page
• Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems: • See our immunoprecipitation selection table, data, and references • Dynabeads magnetic beads selection guide • See magnets for Dynabeads magnetic beads separations • KingFisher automation protocols • Watch a video about the KingFisher Flex instrument Order Info Shipping Condition: Room Temperature