Invitrogen™ Dynabeads™ M-280 Streptavidin

Description

• Dynabeads M-280 Streptavidin beads are 2.8-μm monosized, monodispersed, superparamagnetic, and hydrophobic beads covalently coupled to streptavidin
• They are blocked with BSA and intended for use in the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands
• The excellent dispersion abilities and the absence of magnetic remanence make the Dynabeads streptavidin products ideal for both manual and automated protocols, including microfluidic system protocols
• Benefits of Dynabeads M-280 Streptavidin beads include: • Performance —high sensitivity and reproducibility secure consistent results • Efficiency —fast binding kinetics and high binding capacity • Flexibility —flexible protocols with gentle and efficient liquid-phase reaction kinetics • Automation ready —easy automation for high-throughput needs on the Kingfisher Purification System Consistency and reproducibility The monolayer of streptavidin leaves most biotin-binding sites available for the binding of free biotin and biotinylated ligands
• The specific and defined surface allows for efficient capture, separation, and downstream handling
• In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results
• High binding capacity The size of the ligand and the biotinylation procedure will affect the binding capacity
• The capacity also depends on steric availability and charge interaction between bead and ligand and between ligands
• With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads M-280 Streptavidin typically binds: • 490-750 pmol free biotin • 10 μg biotinylated IgG • ∼200 pmol biotinylated peptides • ∼10 μg biotinylated ds-DNA • ∼200 pmol biotinylated ss-oligonucleotides Applications Thermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications
• Although the beads can be used to isolate any biotinylated targets, these hydrophobic beads are particularly well suited for immunoassays, protein purification, cell isolation, phage display, and biopanning
• Automation ready with the KingFisher Purification System The 2.8-μm magnetic beads are ideal for high-throughput enrichment
• This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers
• Commercial supply Our manufacturing sites are ISO 13485-certified
• Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply
• If you are in the process of customizing Dynabeads M-280 Streptavidin beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page
• Order Info Shipping Condition: Room Temperature