Invitrogen™ Dynabeads™ Sheep Anti-Rat IgG

Description

• Dynabeads™ Sheep Anti-Rat IgG are 4.5 μm superparamagnetic beads with affinity purified polyclonal sheep anti-rat IgG covalently bound to the bead surface
• These beads can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed
• Dynabeads™ Sheep Anti-Rat IgG are especially well suited for cell isolation from mouse samples (cross-reactivity to mouse antibodies is high and cross-reactivity to human antibodies is minimal)
• • Works with any cell type, from any sample and from any species (except rat) • Fast and efficient depletion or positive isolation of any target cell for molecular downstream assays using rat primary antibody • Obtain pure and untouched cells using a mixture of rat IgGs to deplete unwanted cells Magnetic bead-based separation offers easy handling The rat primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads™ Sheep Anti-Rat IgG (direct technique)
• Regardless of which of these techniques is used, when the cell sample and the Dynabeads™ Sheep Anti-Rat IgG are mixed, the beads bind to the target cells
• Placing the sample on a magnet separates the bead-bound cells from the rest of the sample
• One or more rat primary antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for molecular downstream experiments (positive isolation)
• For positive isolation of bead-free cells (e.g., for flow cytometry), we recommend using your rat antibody in combination with the Dynabeads™ FlowComp™ Flexi Kit
• Useful for many sample types Using Dynabeads™ Sheep Anti-Rat IgG, you can isolate cells from many starting samples including whole blood, mouse spleen or lymph node cells, and tissue digests
• Order Info Shipping Condition: Room Temperature