Invitrogen™ Dynabeads™ M-450 Tosylactivated

Description

• Dynabeads™ M-450 Tosylactivated are 4.5 μm superparamagnetic beads containing surface tosyl groups
• The beads covalently bind primary amino and sulfhydryl groups in antibodies to position them in the optimal orientation for cell-surface protein binding
• Low background and covalent binding of the antibodies make these beads an excellent choice for cell separation applications when a downstream release from the beads is not required
• • Quickly deplete cells or positively isolate target cells for downstream molecular analysis • Excellent for cell isolation procedures using your own unique antibody • Several antibodies can be coupled on the same beads for non-cell-based applications, such as cell activation Coupling procedure Covalent coupling is performed overnight by incubating the antibody of choice with the Dynabeads™ M-450 Tosylactivated beads
• Optimal coupling occurs at high pH (8.5–9.5) and at 37°C
• For pH-labile antibodies, coupling can be performed in an alternative buffer at pH 7.4
• Not recommended for immunoprecipitation (IP) These 4.5 μm Tosylactivated beads are not recommended for IP as they have a lower surface capacity per unit mass when compared to 1 μm and 2.8 μm Tosylactivated and Epoxy Dynabeads™
• Order Info Shipping Condition: Room Temperature