Invitrogen™ Dynabeads™ M-450 Epoxy

Description

• Dynabeads™ M-450 Epoxy beads are ideal for custom cell-isolation protocols upstream of molecular applications
• Purified antibodies can be used in conjunction with these beads to create a magnetic cell separation media with the good antibody orientation
• Ideal for Cell Separation when you have a custom antibody: Low background and covalent linking of antibodies to the bead surface make these beads an excellent choice for cell separation applications when a downstream release from the beads is not required
• Ultra rapid protocols: • Quickly isolate targeted cell populations upstream of molecular analyses • Excellent for cell isolation procedures that require custom antibodies • Couple different custom antibodies to different beads to select specific subpopulations of cells Not recommended for Immunoprecipitation (IP): These 4.5 micron beads are not recommended for IP as they have a lower surface capacity per unit mass when compared to 1 μm and 2.8 μm Tosylactivated and Epoxy coated Dynabeads™
• Use rather Dynabeads Protein A (10001D, 10002D), Dynabeads Protein G (10003D, 10004D) or one of the Dynabeads Immunoprecipitation Kits (10006D, 10007D) Coupling procedure outline: Any purified antibody can be covalently coupled to the bead surface in a easy-to-perform overnight reaction
• Simply incubate the antibody you wish to use for your cell isolation procedure with these 4.5 μm Dynabeads™
• Optimal coupling occurs at high pH (8.5-9.5) and at 37°C
• For pH labile antibodies, coupling can be performed in an alternative buffer at pH 7.4
• About Dynabeads™: Dynabeads™ are non-porous monodisperse superparamagnetic beads
• They are highly mobile in solution enabling antibodies coupled to the beads to continuously interact with the cell suspension
• These 4.5 μm superparamagnetic beads experience a strong pull when placed in a magnetic field and gently pull targeted cells to the tube walls when the tube is transferred to a rack containing a strong magnetic field
• With the beads and attached cells concentrated at the tube wall the supernatant liquid containing non-targeted cells is easily and quickly decanted or pipetted away
• Washing steps are performed similarly
• Bead Characteristics for Dynabeads™ M-450 Epoxy: • Coated in glycidyl ether (Epoxy) groups • Hydrophobic, pH Neutral • Covalent binding by primary amine (NH2) or sulphydryl (SH) groups • Large bead 4.5 μm bead size is ideal for cellular isolations • Large bead 4.5 μm bead size not recommended for IP applications Order Info Shipping Condition: Room Temperature