Invitrogen™ Dynabeads™ CD19 Pan B

Description

• Dynabeads™ CD19 Pan B are superparamagnetic beads covalently coupled with an anti-human CD19 antibody that enable easy isolation or depletion of human CD19 + B cells directly from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests
• Advantages of Dynabeads™ CD19 Pan B: • Fast and efficient isolation of CD19 + B cells from any sample—no columns required • High purity, yield, and viability of positively isolated CD19 + cells for molecular downstream assays or efficient depletion of CD19 + B cells • Fast and efficient depletion of CD19 + T cells from any sample About Dynabeads™ CD19 Pan B Dynabeads™ CD19 pan B are uniform (4.5 μm diameter) superparamagnetic beads coated with a primary monoclonal antibody specific for the CD19 membrane antigen mainly expressed on human B cells
• Because of the bead size, Dynabeads™ CD19 pan B can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes
• Dynabeads™ CD19 pan B will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified
• Note that intact cells will not be released from these beads, so if you want to isolate CD19 + cells for cell-based applications, or you need to check your samples with flow cytometry, you can combine with DETACHaBEAD™ CD19 (Cat
• No
• 12506D) (purchased separately to get bead- and antibody-free cells) or use Dynabeads™ Untouched™ Human B Cells (Cat
• No
• 11351D) to obtain untouched human B cells
• Magnetic bead-based separation offers easy handling Dynabeads™ CD19 Pan B is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells
• By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes
• For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells
• For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity
• The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis
• Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests
• Order Info Shipping Condition: Room Temperature