Invitrogen™ Dynabeads™ CD45

Description

• Dynabeads™ CD45 are superparamagnetic beads covalently coupled to anti-human CD45 antibody that enable isolation or depletion of CD45 + leucocytes directly from whole blood, buffy coat, or MNC suspensions
• Dynabeads™ CD45 can also be used to enrich epithelial tumor cells
• The CD45 antigen is weakly expressed on myeloid cells
• To efficiently deplete or isolate all leucocytes including myeloid cells, use Dynabeads™ CD15 (Cat
• No
• 11137D) in combination with Dynabeads™ CD45
• Advantages of Dynabeads™ CD45: • Efficient depletion of human leucocytes • Isolation of leucocytes directly from whole blood for molecular applications • Enrichment of circulating epithelial tumor cells About Dynabeads™ CD45 Dynabeads™ CD45 are uniform, superparamagnetic beads (4.5 μm diameter) coated with a primary monoclonal mouse IgG 2a antibody specific for a CD45 membrane antigen common to all known isoforms of CD45
• CD45 is expressed on all human leucocytes
• Because of the bead size, Dynabeads™ CD45 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes
• Dynabeads™ CD45 is often used for enrichment of circulating non-hematopoietic tumor cells, by depletion of all CD45 + leucecytes from a MNC sample
• Positively isolated cells can be used for downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified
• Note that intact cells will not be released from these beads, and we do not recommend to analyze the bead-bound cells with a flow cytometer
• Magnetic bead–based separation offers easy handling Dynabeads™ CD45 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells
• By placing the sample on a magnet it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes
• For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells
• For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity
• The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis
• Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests
• Order Info Shipping Condition: Room Temperature