Gibco™ Dynabeads™ Human Treg Expander

Description

• Dynabeads Human Treg Expander beads are 4.5-μm superparamagnetic beads covalently coupled with an optimized combination of anti-human CD3 and anti-human CD28 antibodies and intended for ex vivo activation and expansion/proliferation of human regulatory T cells (Tregs)
• Benefits of Dynabeads Human Treg Expander beads include: • Easy —activate and expand Tregs without using antigens or antigen-presenting cells • Efficient —reproducible method and high expansion rate • Retain Tregs —restimulation with beads after 2 weeks increases the expansion and avoids overgrowth of non-Tregs • Functional —expanded Tregs retain their phenotype and suppressive function Simple and fast procedure The Dynabeads Human Treg Expander beads offer a simple method for the activation and expansion of Tregs
• Pre-isolated Tregs are activated by adding the beads and human recombinant IL-2 (rIL-2) to the cell culture
• Optimal expansion is performed when appropriate T-cell medium is used, such as CTS OpTmizer T Cell Expansion SFM (Cat
• No
• A1048501)
• Activation of human Tregs Short time activation : activation of Tregs with Dynabeads Human Treg Expander beads from a few hours to a few days
• This procedure does not expand the cells, and proteomics expression/detection and nucleic acid assays can be performed
• The cell to bead complexes cannot be used in flow cytometry in this phase
• Long-term activation/expansion : activation of Tregs with Dynabeads Human Treg Expander beads for three days to a few weeks to obtain expansion of Tregs
• When experiencing reduced expansion or cell shrinking, the Tregs can be restimulated with the Dynabeads Human Treg Expander beads
• The beads can be removed with a DynaMag magnet and used in cell:cell interaction studies, flow cytometry, proteomics, and nucleic acid-based assays
• Pre-isolate the human Tregs Pure Tregs are important to obtain a good Treg population, and this can be achieved by cell sorting or pre-isolating the Tregs with our Dynabeads Regulatory CD4 + CD25 + T Cell Kit (Cat
• No
• 11363D)
• Alternative activation products, from mouse to clinic • For ex vivo activation and expansion of human T cells for cell-based therapy, use the aseptically manufactured CTS Dynabeads CD3/CD28 (Cat
• No
• 40203D) • For activation of human polyclonal T cells, use Dynabeads Human T-Activator CD3/CD28 (Cat
• No
• 11131D) • For activation of human antigen-specific T cells, use Dynabeads Human T Activator CD3/CD28/CD137 (Cat
• No
• 11162D) • For activation of mouse T cells, use Dynabeads Mouse T Activator CD3/CD28 (Cat
• No
• 11452D) Commercial supply Our manufacturing sites are ISO 13485–certified
• Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility, making them an ideal choice for commercial supply
• If you are in the process of customizing Dynabeads Human Treg Expander beads in a commercial product or service, or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page
• Order Info Shipping Condition: Room Temperature