Invitrogen™ Dynabeads™ M-270 Epoxy

Description

• Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups
• The beads covalently bind primary amino and sulfhydryl groups in proteins and peptides, making them ideal for coupling antibodies, peptides, intact proteins, and functional enzymes
• These hydrophylic, neutral pH beads exhibit extremely low nonspecific binding of proteins and dyes, which reduces the need for blocking agents
• Also, Dynabeads™ M-270 Epoxy Beads do not contain Tween™ detergent, and so are ideal for use in mass spectrometry (MS) analysis
• • Ideal for immunoprecipitation (IP) of proteins and protein complexes • Gentle, yet rapid magnetic separation and short incubation times allow identification of transient and labile complexes • Covalent antibody-coupling to the Dynabeads™ avoids co-elution of the antibody with the target protein Adaptable for various applications Fast surface binding kinetics and rapid magnetic separation characteristics make Dynabeads™ M-270 Epoxy beads the ideal choice for applications such as immunoprecipitation/purification of proteins and protein complexes, coupling functional enzymes to the bead surface for downstream assays, and identifying protein binding partners
• Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag™ magnet, and the magnetic separation properties of the Dynabeads™
• Coupling procedure Covalent coupling is performed overnight by incubating the desired ligand (commonly antibodies, peptides, or proteins) with Dynabeads™ M-270 Epoxy beads
• The binding capacity varies depending on the ligand used, but is typically 5–10 μg IgG per milligram of beads
• Coupling occurs at neutral pH and high salt concentration
• The coupling reaction can be performed over a broad range of temperatures to match the stability characteristics of your ligand
• Upon completion of the ligand coupling step, the reactive moieties on the Dynabeads™ M-270 Epoxy beads are deactivated to prevent further coupling
• Once the coupling reaction is completed, these nonporous, hydrophilic, pH neutral beads exhibit extremely low nonspecific binding
• Order Info Shipping Condition: Room Temperature