Invitrogen™ Dynabeads™ M-280 Tosylactivated

Description

• When you wish to covalently couple antibodies, peptides, intact proteins and functional enzymes to the surface of a magnetic bead choose Dynabeads Tosylactivated
• Optimal antibody orientation makes these beads an excellent choice for the immunoprecipitation of proteins and protein complexes
• Ideal for Immunoprecipitation of protein complexes: Low background and covalent linking of antibodies to the bead surface make Dynabeads Tosylactivated an excellent choice for Immunoprecipitation of proteins and protein complexes (Co-Immunoprecipitation, Co-IP)
• Gentle and rapid magnetic concentration of the beads and short incubation times (possible due to fast surface-based binding kinetics) make Dynabeads Tosylactivated an excellent choice for the immunoprecipitation of highly-labile or and/or transient (short-lived) protein complexes
• Ultra rapid protocols: • Identify members of protein complexes in minutes rather than hours • Temporal resolution short enough to identify transient and labile complexes • Identify binding partners that cannot be identified with longer protocols • Rapid protocols further reduce the already ultra-low background binding • Stronger signal to noise-ratios Applications: • IP of proteins and protein complexes • Couple functional enzymes to bead surface for downstream assays • Couple peptides to bead surface to identify binders Purify peptides, proteins and enzymes that are: • Transiently stable • Structurally intact • Temperature labile • In their native conformation and functional Coupling Procedure Outline: Covalent coupling is performed overnight by incubating the desired ligand with the Dynabeads Tosylactivated
• Ligands commonly coupled to Dynabeads Tosylactivated include peptides and proteins (e.g
• antibodies for Immunoprecipitation or Co-Immunoprecipitation)
• Coupling occurs at neutral to high pH and at 37°C
• We recommend coupling at pH 8.5–9.5, but for pH labile ligands, coupling can be performed in an alternative buffer at pH 7.4
• Upon completion of the ligand coupling step, the actual Dynabeads Tosylactivated surface coating will be rendered inert, resulting in low non-specific binding
• About Dynabeads: Dynabeads are non-porous monodisperse superparamagnetic beads
• They are highly mobile in solution enabling ligands coupled to the beads to continuously interact with the entire sample volume
• The superparamagnetic beads are pulled to the tube walls by transferring the tube to a rack containing a strong magnetic field (From the red navigation bar navigate Brands > Dynal > Magnets)
• Strong magnetic fields quickly pull the beads to the tube wall, allowing for easy and complete removal of the supernatant by pipette
• Washing steps are performed similarly.l Bead Surface Characteristics for Dynabeads Tosylactivated: • p-toluene-sulfonyl (Tosyl) groups • Hydrophobic, pH Neutral • Covalent binding by primary amine (NH 2 ) or sulphydryl (SH) groups Binding capacity per milligram beads: Varies depending on ligand, (e.g
• 5–10 μg IgG)