3dGRO® Human iPSC Derived Colon Organoids

Description

• General description: Human intestinal organoids are self-organizing, 3D structures that can be expanded in long-term culture and differentiate into intestinal epithelial cell types. Epithelial intestinal organoids, often referred to as enteroids or “mini-guts”, maintain the physiological characteristics of the gastrointestinal system and have been a useful cell culture tool to model intestinal development and disease including the study of colon cancer, celiac disease, inflammatory bowel diseases and host microbiome interactions. Organoids can be propagated from patient biopsies and from embryonic (ES) and induced pluripotent stem cells (iPS cells).3dGRO® Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1.). The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. 3dGRO® human iPSC derived colon organoids can be propagated long-term by passaging every 10-12 days using 3dGRO® Human Colon Organoid Expansion Medium (Cat. No. SCM304).
• Application: Highly characterized cryopreserved human colon intestinal organoids derived from integration-free human induced pluripotent stem cells (iPSCs).Research Category:Cell CultureStem Cell Research
• Quality: Each vial contains ≥1500 organoids.Mycoplasma: NegativeGenotyped by STR analysis: PassHuman species verifiedOrganoids are negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.Cell viability test: Pass
• Storage and Stability: 3dGRO® Human iPSC Derived Colon Organoids should be stored in liquid nitrogen.
• Legal Information: 3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.