Interleukin-6 human

Description

• General description: IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130).[1] The gene IL-6 is mapped to human chromosome 7p15.3.[2] Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kDa protein containing 184 amino acid residues.
• Application: IL-6 human has been used for STAT3 (signal transducer and activator of transcription 3) activation.[3] It has also been used as a standard for detection of IL-6 in Ben-Men-I cells (human WHO grade I meningioma derived, hTERT immortalized cells).[4]
• Biochem/physiol Actions: IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism.[1] IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma.[1]
• Packaging: 100 μg
• Sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
• Physical form: Lyophilized from a 0.2 um filtered solution of phosphate buffered saline (PBS), pH 7.4, and stabilizers.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute the contents using endotoxin free water to a concentration of 100 ug/mL. For lower concentrations, the product should be diluted before use in medium containing at least 0.5% protein (i.e., cell culture medium containing 5–10% serum).