RANTES human

Description

• General description: Regulated on activation, normal T cell expressed and secreted (RANTES) also known as C-C motif chemokine ligand 5 (CCL5) is encoded by the gene mapped to human chromosome 17.[1] RANTES belongs to intercrine or chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections.[2][3] Recombinant human RANTES is a 7.8kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
• Application: RANTES human has been used in following studies:In transmigration experiments to determine whether LFA-1 cross-linking could affect peripheral blood T lymphocytes (PBTL) migration in response to the chemokine RANTES.[4]Luminol-dependent chemiluminescence assay to determine the effect of RANTES on reactive oxygen species (ROS) production by eosinophils.[5]
• Biochem/physiol Actions: Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4+/CD45RO), basophils, and eosinophils.[3] The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5.1 RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. The In1.1C variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV-1 infection.[1][6]
• Physical form: Lyophilized with no additives.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.