Peroxisome Isolation Kit

Description

• General description: Isolated peroxisomes are used for studying lipid β-oxidation,[1] amino acid metabolism[2] and biosynthesis of ether-linked glycerolipids[3] and bile acids.[4][5]
• Application: The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.
• Features and Benefits: Specially formulated extraction reagents for research scale applications - save time and minimize wasteProduces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysisCompatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)
• Other Notes: Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.
• Legal Information: OptiPrep is a trademark of Serumwerk Bernburg AG