TOX7
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
Manufacturer: Sigma Aldrich
Synonym(S): LDH cytotoxicity assay
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 KIT | TOX7-1-KIT | In Stock | ₹ 41,558.40 |
TOX7 - 1 KIT
In Stock
Quantity
1
Base Price: ₹ 41,558.40
GST (18%): ₹ 7,480.512
Total Price: ₹ 49,038.912
form
liquid
Quality Level
200
usage
kit sufficient for 500 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
protein quantification: suitable
λmax
490 nm
application(s)
cell analysisdetection
detection method
colorimetric
storage temp.
−20°C
Related Products
Description
- General description: Tox7 is a member of Sigma′s CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays, as well as conventional cytotoxicity resulting from interaction of a test material with the cell.
- Application: In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based has been used:to detect lactate dehydrogenase release in human umbilical vein endothelial cells (HUVECs) and human cardiac myocytes (HCMs) for lactate dehydrogenase (LDH) assay[1]to determine the activity of lactate dehydrogenase leakage of MDA-MB-231 cells[2]to examine the activity of LDH in RAW 264.7 cells [3], in LDH cytotoxicity assay in human cell lines[4]for spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 490 nm.
- Biochem/physiol Actions: The lactate dehydrogenase assay is a means of measuring either the number of cells via total cytoplasmic lactate dehydrogenase (LDH) or membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. This method is simple, accurate and yields reproducible results. The assay is based on the reduction of NAD by LDH. The resulting reduced NAD (NADH) is utilized in the stoichiometric conversion of a tetrazolium dye to a soluble, colored formazan derivative..
SAFETY INFORMATION
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
WGK
WGK 3
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form: liquid
Quality Level: 200
usage: kit sufficient for 500 tests
packaging: pkg of 1 kit
storage condition: dry at room temperature
technique(s): protein quantification: suitable
λmax: 490 nm
application(s): cell analysisdetection
detection method: colorimetric
storage temp.: −20°C
form:
liquid
Quality Level:
200
usage:
kit sufficient for 500 tests
packaging:
pkg of 1 kit
storage condition:
dry at room temperature
technique(s):
protein quantification: suitable
λmax:
490 nm
application(s):
cell analysisdetection
detection method:
colorimetric
storage temp.:
−20°C
form: liquid
Quality Level: 200
usage: kit sufficient for 2,000 tests
packaging: pkg of 1 kit
storage condition: dry at room temperature
technique(s): protein quantification: suitable
λmax: __
application(s): cell analysisdetection
detection method: colorimetricfluorometric
storage temp.: 2-8°C
form:
liquid
Quality Level:
200
usage:
kit sufficient for 2,000 tests
packaging:
pkg of 1 kit
storage condition:
dry at room temperature
technique(s):
protein quantification: suitable
λmax:
__
application(s):
cell analysisdetection
detection method:
colorimetricfluorometric
storage temp.:
2-8°C
form: __
Quality Level: 200
usage: __
packaging: __
storage condition: __
technique(s): __
λmax: __
application(s): __
detection method: __
storage temp.: 2-8°C
form:
__
Quality Level:
200
usage:
__
packaging:
__
storage condition:
__
technique(s):
__
λmax:
__
application(s):
__
detection method:
__
storage temp.:
2-8°C
form: __
Quality Level: __
usage: __
packaging: __
storage condition: __
technique(s): __
λmax: __
application(s): __
detection method: __
storage temp.: __
form:
__
Quality Level:
__
usage:
__
packaging:
__
storage condition:
__
technique(s):
__
λmax:
__
application(s):
__
detection method:
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storage temp.:
__