TOX1
In Vitro Toxicology Assay Kit, MTT based
Manufacturer: Sigma Aldrich
Synonym(S): mitochondrial activity assay
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 KIT | TOX1-1-KIT | In Stock | ₹ 49,905.60 |
TOX1 - 1 KIT
In Stock
Quantity
1
Base Price: ₹ 49,905.60
GST (18%): ₹ 8,983.008
Total Price: ₹ 58,888.608
usage
kit sufficient for 1,000 tests
Quality Level
200
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
570 nm
application(s)
cell analysisdetection
detection method
colorimetric
storage temp.
2-8°C
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Description
- General description: This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.
- Application: In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP).[1] It has also been used to examine cell viability[2] in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.[3]
- Biochem/physiol Actions: Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3
Target Organs
Central nervous system, Respiratory system
WGK
WGK 3
Flash Point(F)
53.6 °F
Flash Point(C)
12 °C
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usage: kit sufficient for 1,000 tests
Quality Level: 200
packaging: pkg of 1 kit
storage condition: dry at room temperature
λmax: 570 nm
application(s): cell analysisdetection
detection method: colorimetric
storage temp.: 2-8°C
usage:
kit sufficient for 1,000 tests
Quality Level:
200
packaging:
pkg of 1 kit
storage condition:
dry at room temperature
λmax:
570 nm
application(s):
cell analysisdetection
detection method:
colorimetric
storage temp.:
2-8°C
usage: kit sufficient for 1,000 tests
Quality Level: 200
packaging: pkg of 1 kit
storage condition: dry at room temperature
λmax: 540 nm
application(s): cell analysisdetection
detection method: colorimetric
storage temp.: 2-8°C
usage:
kit sufficient for 1,000 tests
Quality Level:
200
packaging:
pkg of 1 kit
storage condition:
dry at room temperature
λmax:
540 nm
application(s):
cell analysisdetection
detection method:
colorimetric
storage temp.:
2-8°C
usage: kit sufficient for 500 tests
Quality Level: 200
packaging: pkg of 1 kit
storage condition: dry at room temperature
λmax: 490 nm
application(s): cell analysisdetection
detection method: colorimetric
storage temp.: −20°C
usage:
kit sufficient for 500 tests
Quality Level:
200
packaging:
pkg of 1 kit
storage condition:
dry at room temperature
λmax:
490 nm
application(s):
cell analysisdetection
detection method:
colorimetric
storage temp.:
−20°C
usage: kit sufficient for 2,000 tests
Quality Level: 200
packaging: pkg of 1 kit
storage condition: dry at room temperature
λmax: __
application(s): cell analysisdetection
detection method: colorimetricfluorometric
storage temp.: 2-8°C
usage:
kit sufficient for 2,000 tests
Quality Level:
200
packaging:
pkg of 1 kit
storage condition:
dry at room temperature
λmax:
__
application(s):
cell analysisdetection
detection method:
colorimetricfluorometric
storage temp.:
2-8°C