EX-CELL® 302 Serum-Free Medium for CHO Cells

Description

• General description: EX-CELL® 302 is a serum-free liquid medium, which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL® 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System™, because it does not contain hypoxanthine, thymidine or L-glutamine. If EX-CELL® 302 is to be used with CHO cells transformed using the GS selection the media needs to be supplemented with GS Supplement 50X (Catalog No. 58672C) for the additional amino acids and nucleosides. For use with DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL® 302 was developed using only recombinant human proteins that have molecular weights less than 10 kD. The total protein concentration found in EX-CELL® 302 is less than 1 mg/L. Pluronic® F68 has been added at a final concentration of 0.1% to EX-CELL® 302 to protect against shear damage in sparged bioreactor systems. Catalog No. 14326C replaces Catalog No. 14313 and includes an alternate source of soy hydrolysate to that found in the original EX-CELL® 302 formulation. With more consistent performance and improved filtration characteristics, the alternate hydrolysate will improve the overall performance and consistency of EX-CELL® 302. Comparability testing utilizing the previous soy hydrolysate and the replacement hydrolysate demonstrated comparable growth promoting characteristics.
• Caution: Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
• Legal Information: EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany