M0650

Medium 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture, 10X

Manufacturer: Sigma Aldrich

Synonym(S): M199 Medium, TCM 199

Select a Size

Pack Size SKU Availability Price
100 ML M0650-100-ML In Stock ₹ 3,788.75

M0650 - 100 ML

₹ 3,788.75

In Stock

Quantity

1

Base Price: ₹ 3,788.75

GST (18%): ₹ 681.975

Total Price: ₹ 4,470.725

Quality Level

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: nophenol red: yessodium pyruvate: noHEPES: noEarle’s salts (5% CO2): yesL-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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Description

  • General description: Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
  • Application: Medium 199 has been used:to culture human mesothelial cells[1]as a component of the holding medium for the collection of embryos from pluriparous Boer goat[2]in the preparation of spheroid-embedded collagen matrices[3]
  • Reconstitution: Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

SAFETY INFORMATION

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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