M5650

Minimum Essential Medium Eagle

With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

Manufacturer: Sigma Aldrich

Synonym(S): EMEM, MEM

Select a Size

Pack Size SKU Availability Price
500 ML M5650-500-ML In Stock ₹ 4,449.08
1 L M5650-1-L In Stock ₹ 7,425.95

M5650 - 500 ML

₹ 4,449.08

In Stock

Quantity

1

Base Price: ₹ 4,449.08

GST (18%): ₹ 800.834

Total Price: ₹ 5,249.914

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yesNaHCO3: yesL-glutamine: nosodium pyruvate: noHEPES: noEarle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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Description

  • General description: Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.
  • Application: Minimum Essential Medium Eagle has been used to culture:HeLa cells[1]Madin-Darby canine kidney (MDCK) cells[2]chondrosarcoma cell line[1]This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
  • Reconstitution: Supplement with 0.292 g/L L-glutamine.

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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