M2520
Medium 199
HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): M199 Medium, TCM 199
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 X 1 L | M2520-10-X-1-L | In Stock | ₹ 26,683.63 |
M2520 - 10 X 1 L
In Stock
Quantity
1
Base Price: ₹ 26,683.63
GST (18%): ₹ 4,803.053
Total Price: ₹ 31,486.683
Quality Level
500
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yesphenol red: yesEarle’s salts (5% CO2): yesNaHCO3: nosodium pyruvate: noHEPES: 25 mM
shipped in
ambient
storage temp.
2-8°C
Related Products
Description
- General description: Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
- Application: Medium 199 has been used to:maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation[1]to rinse the aspiration line during the collection of the ovum from German Holstein cows[2]to culture cardiac endothelial cells from rats[3]
- Quantity: Formulated to contain 14.7 grams of powder per liter of medium.
- Reconstitution: Supplement with 2.2 g/L sodium bicarbonate.
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Show Difference
Quality Level: 500
form: powder
technique(s): cell culture | mammalian: suitable
components: L-glutamine: yesphenol red: yesEarle’s salts (5% CO2): yesNaHCO3: nosodium pyruvate: noHEPES: 25 mM
shipped in: ambient
storage temp.: 2-8°C
Quality Level:
500
form:
powder
technique(s):
cell culture | mammalian: suitable
components:
L-glutamine: yesphenol red: yesEarle’s salts (5% CO2): yesNaHCO3: nosodium pyruvate: noHEPES: 25 mM
shipped in:
ambient
storage temp.:
2-8°C
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shipped in:
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form: solid
technique(s): __
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shipped in: __
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__
shipped in:
__
storage temp.:
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