M3769

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Manufacturer: Sigma Aldrich

Synonym(S): M199 Medium, TCM 199

Select a Size

Pack Size SKU Availability Price
10 X 1 L M3769-10-X-1-L In Stock ₹ 7,458.43
50 L M3769-50-L In Stock ₹ 23,793.35

M3769 - 10 X 1 L

₹ 7,458.43

In Stock

Quantity

1

Base Price: ₹ 7,458.43

GST (18%): ₹ 1,342.517

Total Price: ₹ 8,800.947

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: nophenol red: noHEPES: nosodium pyruvate: noEarle’s salts (5% CO2): yesNaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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Description

  • General description: Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
  • Application: Medium 199 has been used:to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assayto flush the uterine horn of gilts to recover day 12 conceptuses from uterito culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)
  • Quantity: Formulated to contain 9.4 grams of powder per liter of medium.
  • Reconstitution: Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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