M7653
Medium 199
With Hanks′ salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): M199 Medium, TCM 199
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 ML | M7653-500-ML | In Stock | ₹ 6,191.90 |
| 6 X 500 ML | M7653-6-X-500-ML | In Stock | ₹ 30,201.75 |
M7653 - 500 ML
In Stock
Quantity
1
Base Price: ₹ 6,191.90
GST (18%): ₹ 1,114.542
Total Price: ₹ 7,306.442
Quality Level
400
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: noNaHCO3: yesHanks’ salts (2% CO2): yessodium pyruvate: nophenol red: yesHEPES: no
shipped in
ambient
storage temp.
2-8°C
Related Products
Description
- General description: Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
- Reconstitution: Supplement with 0.1 g/L L-glutamine.
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Compare Similar Items
Show Difference
Quality Level: 400
sterility: sterile-filtered
form: liquid
technique(s): cell culture | mammalian: suitable
impurities: endotoxin, tested
components: L-glutamine: noNaHCO3: yesHanks’ salts (2% CO2): yessodium pyruvate: nophenol red: yesHEPES: no
shipped in: ambient
storage temp.: 2-8°C
Quality Level:
400
sterility:
sterile-filtered
form:
liquid
technique(s):
cell culture | mammalian: suitable
impurities:
endotoxin, tested
components:
L-glutamine: noNaHCO3: yesHanks’ salts (2% CO2): yessodium pyruvate: nophenol red: yesHEPES: no
shipped in:
ambient
storage temp.:
2-8°C
Quality Level: 200
sterility: __
form: buffered aqueous glycerol solution
technique(s): flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells.immunocytochemistry: 1:1,000 using HeLa cellsimmunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinomaimmunoprecipitation (IP): 1:50western blot: 1:1,000 using serum-treated NIH-3T3 cells
impurities: __
components: __
shipped in: __
storage temp.: __
Quality Level:
200
sterility:
__
form:
buffered aqueous glycerol solution
technique(s):
flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells.immunocytochemistry: 1:1,000 using HeLa cellsimmunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinomaimmunoprecipitation (IP): 1:50western blot: 1:1,000 using serum-treated NIH-3T3 cells
impurities:
__
components:
__
shipped in:
__
storage temp.:
__
Quality Level: 200
sterility: __
form: crystals
technique(s): __
impurities: __
components: __
shipped in: __
storage temp.: __
Quality Level:
200
sterility:
__
form:
crystals
technique(s):
__
impurities:
__
components:
__
shipped in:
__
storage temp.:
__
Quality Level: 200
sterility: __
form: powder
technique(s): HPLC: suitable
impurities: __
components: __
shipped in: __
storage temp.: room temp
Quality Level:
200
sterility:
__
form:
powder
technique(s):
HPLC: suitable
impurities:
__
components:
__
shipped in:
__
storage temp.:
room temp