Description

• General description: Thiamine or Vitamin B1 is a positively charged and essential constituent for all tissues. It is present at higher levels in skeletal muscles, liver, kidney, heart, and brain.[1] Thiamine is composed of a pyrimidine ring linked to a thiazole ring via a methylene bridge. [2]
• Application: Thiamine hydrochloride has been used as a component:of the vitamin-concentrated stock solution for the preparation of Hv-Ca medium for culturing Haloferax volcanii[3]of the tris-acetate-phosphate (TAP) medium for culturing Chlamydomonas nivalis[4]of modified Bold 3N medium for culturing Chlorella minutissima[5]
• Biochem/physiol Actions: Thiamine is converted to thiamine diphosphate post-movement into the cellular membranes by thiamine diphosphokinase.[1] Thiamine diphosphate, an active cofactor is essential for the mechanism of many enzymes involved in the citric acid cycle, the glycolytic pathway, the degradation of branched-chain amino acids, and the pentose phosphate pathway.[6] Thiamine is involved in converting food into energy required for functioning the central and peripheral nervous systems. Lower levels of this vitamin are associated with delirium, Wernicke′s encephalopathy, and Wernicke-Korsakoff syndrome.[7] Thiamine hydrochloride is required to support energy metabolism and amino acid synthesis in cultured cells. It is present in many classical and serum-free formulations.