Description

• General description: Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.
• Application: Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells[3]mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization[4]blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence[5]
• Biochem/physiol Actions: Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation.[6] It aids the transportation of drugs, hormones, and fatty acids.[1] BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[7]
• Warning: Toxicity: Standard Handling (A)
• Preparation Note: May take up to 24 h to fully dissolve.
• Reconstitution: Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany