Rat Collagen Type IV
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation
Manufacturer: Sigma Aldrich
CAS Number: 9007-34-5
Synonym(S): Type IV collagen, EHS-collagen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 VIAL | C0543-1-VIAL | In Stock | ₹ 32,964.40 |
C0543 - 1 VIAL
In Stock
Quantity
1
Base Price: ₹ 32,964.40
GST (18%): ₹ 5,933.592
Total Price: ₹ 38,897.992
product name
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
biological source
mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
Quality Level
200
type
Type IV (Miller)
product line
BioReagent
form
lyophilized powder
packaging
glass bottle of 1 vial
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
solubility
0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
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Description
- General description: Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma.[1] [2]The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.
- Application: Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used: in adhesion assays.[3]in hydrogel fabrication.[4] to coat cell culture plates for cell differentiation.[5] to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.[6]
- Biochem/physiol Actions: Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules.[1] It is also used in 2D coatings.[7]
- Other Notes: Native collagen
- Quantity: 0.75 mg/vial
- Preparation Note: Chloroform treated.Pepsin is not used in the preparation.
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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product name: Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
biological source: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
Quality Level: 200
type: Type IV (Miller)
product line: BioReagent
form: lyophilized powder
packaging: glass bottle of 1 vial
technique(s): cell culture | mammalian: suitable
surface coverage: 6‑10 μg/cm2
solubility: 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
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Quality Level: 200
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form: buffered aqueous solution
packaging: __
technique(s): flow cytometry: 5 μL using 1 × 106 cells
surface coverage: __
solubility: __
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biological source: bovine heart
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product line: __
form: lyophilized powder
packaging: __
technique(s): __
surface coverage: __
solubility: __