Wnt-5a Protein, Recombinant mouse

Description

• General description: Product Source: Wnt5a has been purified from conditioned media using blue-Sepharose, gel filtration and heparin affinity chromatography.
• Application: • This protein is extremely sticky and will stick to plasticware in the absence of Chaps detergent. • When using this protein, if possible, dose it directly onto cells at the required dilution without pre-filtering. • The toxicity of the Chaps in the storage buffer should be tested empirically on the cell line prior to using the Wnt5a protein. • Generally, we suggest that a minimum dilution of Wnt5a of 1:1000 on mammalian cells results in a final concentration of Chaps that is not toxic to the cells. • If dosing on cells over a period of days/weeks, it is recommended that media containing Wnt5a is replenished daily, rather than dosing Wnt5a to existing media daily. This is because accumulation of Chaps is undesirable for cell viability.
• Packaging: 8-10 μg/vial
• Physical form: Frozen liquid in PBS containing 1.0% Chaps.
• Storage and Stability: Maintain the frozen material at -20 °C until expiration date as stated on the label. Upon receipt, thaw and aliquot into single-use aliquots and store at -20 °C. Once a single-use aliquot has been thawed, DO NOT re-freeze. Store thawed material at 4 °C for up to one month.
• Analysis Note: Specific Activity: The biological activity of Wnt5a has been demonstrated by its ability to antagonize the induction of alkaline phosphatase synthesis by Wnt3a in the presence of BMP4 by C2C12 osteoblastic cells.The EC50 for this effect is typically 100 ng/mL
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.