IL-1β, Human Recombinant Animal Free

Description

• General description: IL-1β is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant human IL-1β is a 17.3 kDa protein containing 153 amino acid residues.
• Application: Research CategoryStem Cell Research
• Quality: Determined its ability to stimulate the proliferation of mouse D10S cells. The expected ED50 is ≤ 0.001 ng/ml, corresponding to a specific activity of ≥ 1 x 10^9 units/mg.
• Physical form: Product is presented in 10mM Sodium Phosphate, pH 7.4 + 50mM NaCl and is filtered through a 0.2 micron filter before lyophilization.
• Storage and Stability: Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.