Lipopolysaccharides from Escherichia coli O111:B4

Description

• Application: Lipopolysaccharides from Escherichia coli O111:B4 has been used:to stimulate an inflammatory response in porcine liver tissues in-vivo[1] and piglet ileum tissues ex vivo[2] to stimulate Ex vivo natural killer(NK) cells[3] and hepatic B cells[4] to induce arthritis in a standard rabbit model[5]
• Biochem/physiol Actions: Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics.[6]
• Preparation Note: The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
• Other Notes: To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.