Normal Mouse Serum

Description

• General description: Normal carrier sera from healthy, non-immunized mice.
• Application: ELISA (see comments)Flow Cytometry (see comments)Immunoblotting (see comments)Immunofluorescence (see comments)Immunohistochemistry (see comments)Immunoprecipitation (see comments)
• Warning: Toxicity: Standard Handling (A)
• Physical form: Lyophilized serum. Lyophilized from 250 mM sodium chloride, 10 mM sodium phosphate, pH 7.6.
• Reconstitution: Reconstitute the lypohilized serum in 1 ml of sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
• Other Notes: Useful as a blocking agent in immunohistochemical procedures or as a negative control for specific mouse antibodies. For use as a negative control in any application, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany