BER human cell line

Description

• General description: Consanguineous: No
• Cell Line Description: This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
• DNA Profile: A: 201Cw: 602B: 1302HLA DRA: 101HLA DRB1: 701HLA DRB4: 101HLA DQAP: 2.1HLA DQA1: 201HLA DQB1: 201HLA DPA1: 1HLA DPB1: 02012 0401
• Serological Profile: HLA A: 2HLA A ief: 2.2HLA C: 6HLA B: 13HLA B ief: 13.2Bw4/bw6: 4HLA Dw: 7HLA DR: 7HLA DR53: 53HLA DQ: 2HLA DP: 2 4
• Culture Medium: RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
• Subculture Routine: Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
• Other Notes: Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
• Disclaimer: This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.