UM-SCC-104 HPV-16 Positive Squamous Carcinoma Cell Line

Description

• General description: All UM-SCC cell lines were derived in the lab of Dr. Thomas Carey at the University of Michigan and are exclusively distributed by Merck KGaA, Darmstadt, Germany. Access to UM-SCC cells is limited solely to those officers, employees and students of PURCHASER’s institution who need access to perform research. PURCHASER may not distribute UM-SCC cells or derivatives to third parties. PURCHASER shall comply with all applicable laws in its use and handling of the Product and shall keep it under reasonable safe and secure conditions to prevent unauthorized use or access.THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS LIMITED USE LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF UM-SCC CELLS, INCLUDING LICENSING TO COMMERCIAL ENTITIES, PLEASE CONTACT: licensing@emdmillipore.comHead and neck squamous-cell carcinoma (HNSCC) is the 6th most common type of cancer world-wide. The cancer may occur in the lip, oral cavity, nasal cavity, paranasal sinuses, salivary glands, pharynx and larynx. Risk factors include smoking, alcohol consumption, betel nut chewing, wood dust exposures and human papilloma virus (HPV) infections. Approximately 15% of HNSCC contain genomic DNA from HPV. In particular, HPV-16 occurs in 90-95% of all HPV-positive HNSCC cases. In oral and pharynx cancer, HPV DNA is found in the tonsils in 45-67% of the cases. HPV DNA is found in 13-25% of the cases in the hypopharynx, 12-18% in the oral cavity and 3-7% in the larynx. UM-SCC-104 is a unique head and neck squamous carcinoma cell line derived from a 56-year old male with a recurrent oral cavity tumor naturally infected with high-risk human papillomavirus (HPV-16). The patient had a history of moderate consumption of alcohol and smoking (20 pack/year). UM-SCC-104 contains a small population of ALDH+ cancer stem cells. The tumor from which the cell line was derived failed to respond to initial chemoradiation therapy and thus UM-SCC-104 is a useful model for preclinical studies of aggressive non-responsive tumors and may be used for studying the interplay between viral and carcinogen-related pathways in tumor progression.UM-SCC-104 was established at the University of Michigan with written informed consent obtained from the patient and with the approval of the study by the Medical School Institutional Review Board.Reference: 1) Carey, T., et al. (2012) Genotyping of 73 UM-SCC head and neck squamous cell carcinoma cell lines. Head Neck. 2010 April; 32(4): 417–426.
• Cell Line Description: Cancer Cells
• Application: Research CategoryInfectious DiseasesInflammation & ImmunologyApoptosis & Cancer
• Components: 1) ≥1X106 viable UM-SCC-104 Squamous Carcinoma Cells: (Catalog No. SCC072). Store in liquid nitrogen.
• Quality: • Each vial contains ≥ 1X10^6 viable cells.• Cells are tested by PCR and are positive for HPV-16 and negative for Hepatitis A, B, C and HIV-1 & 2 viruses.• Cells are negative for mycoplasma contamination.• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
• Storage and Stability: UM-SCC-104 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
• Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.