MS5-mDLL1 Stromal Cell Line

Description

• General description: In vivo, T-cells develop in the thymus from bone-marrow derived hematopoietic stem and progenitor cells (HSPCs). In vitro, various model systems have been employed to mimic thymic function supportive of T-cell development including the OP9-DL1 system. Recently, a serum-free artificial thymic organoid (ATO) system was developed that supports highly efficient and reproducible in vitro differentiation and positive selection of human T-cells from multiple sources of HSPCs, including cord blood, bone marrow and peripheral blood CD34+ HSPCs (Seet et al. 2017). A key component of the ATO system is the MS5-mDLL1 cell line which obviates the need for primary thymic tissues.
• Cell Line Description: Stromal Cells
• Application: Research CategoryImmune ResponseInflammation & Immunology
• Quality: • Each vial contains ≥1X10⁶ viable cells.• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.• Cells are negative for mycoplasma contamination.
• Storage and Stability: Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
• Disclaimer: This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.