N27-A Rat Dopaminergic Neural Cell Line

Description

• General description: N27-A is a clonal derivative of N27 characterized by consistently high expression of the dopaminergic markers TH, dopamine transporter (DAT), and Tuj1 [1]. N27-A cells express the dopaminergic neuron transcription factors Nurr1, En1, FoxA2, and Pitx3 as well as the monoamine transporter VMAT2, but do not express dopamine-beta-hydroxylase (DbH), the enzyme that converts dopamine to norepinephrine. N27-A cells exhibit higher sensitivity to neurotoxins as compared to parental N27 cells, and release dopamine under both basal and depolarization conditions. These features make the N27-A cell line an improved model for Parkinson′s disease research.Source:The N27-A cell line is a clonal derivative of the parental N27 cell line selected for high expression of dopaminergic neural markers. The parental cell line was originally isolated from dopaminergic neurons from an embryonic day 14 rat mesencephalon and immortalized with SV40 large T antigen [2].
• Cell Line Description: Neural Lineage Cells
• Application: N27-A rat dopaminergic neural cell line is a suitable model for Parkinson’s disease research.
• Quality: • Each vial contains ≥ ≥1X10⁶ cells/vial.• Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services.• Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.• Cells are negative for mycoplasma contamination.
• Storage and Stability: Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
• Disclaimer: This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.