Gibco™ MEM α, nucleosides, powder

Description

• Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells
• MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells
• We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This MEM α is modified as follows: With: Ribonucleosides, Deoxyribonucleosides, L-glutamine, Phenol Red Without: Sodium Bicarbonate The complete formulation is available
• Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B 12 , biotin, and ascorbic acid
• MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells
• This product is made with Earle’s salts
• Product Intended Use For in vitro diagnostic use
• CAUTION: Not for human or animal therapeutic use
• Uses other than the intended use may be a violation of local law
• MEM α contains no proteins, lipids, or growth factors
• Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO 2 environment to maintain physiological pH
• Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details)
• Order Info Shipping Condition: Room Temperature