Gibco™ MEM, powder

Description

• Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media
• MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes
• We offer a variety of Gibco™ MEM modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This MEM is modified as follows: With: L-glutamine, Phenol Red Without: HEPES, Sodium Bicarbonate The complete formulation is available
• Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME)
• Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification
• MEM is available with Earle’s salts for use in a CO 2 incubator, or with Hanks' salts for use without CO 2
• This product is made with Earle’s salts
• Using MEM MEM contains no proteins, lipids, or growth factors
• Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO 2 environment to maintain physiological pH
• Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details)
• Order Info Shipping Condition: Room Temperature