Gibco™ Advanced RPMI 1640 Medium

Description

• Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation
• Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology
• i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat
• This Advanced RPMI 1640 is manufactured as follows: With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red Without: L-glutamine, HEPES The complete formulation is available
• Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
• Product Use For Research Use Only
• Not intended for animal or human diagnostic or therapeutic use
• Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement
• Many cell lines do not require adaptation to this media
• The FBS concentration must be optimized for each cell line to obtain maximum serum reduction
• Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO 2 environment to maintain physiological pH
• i Advanced Media: Reduced Sera Solutions
• Quest, 2004
• 1.3:26-30
• Order Info Shipping Condition: Room Temperature Compliance Gibco Advanced RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York
• The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.