Gibco™ Advanced DMEM

Description

• Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation
• Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology
• Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
• This Advanced DMEM is manufactured as follows: With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red Without: L-glutamine The complete formulation is available
• Using Advanced DMEM Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
• Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement
• Many cell lines do not require adaptation to this media
• The FBS concentration must be optimized for each cell line to obtain maximum serum reduction
• Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO 2 environment to maintain physiological pH
• Order Info Shipping Condition: Room Temperature