4919814
Gibco™ Advanced DMEM
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Manufacturer: Fischer Scientific
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Content And Storage
Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Classification
Animal Origin
Form
Liquid
Serum Level
Reduced Serum
With Additives
High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Green Features
Sustainable packaging
Product Line
Gibco™
Cell Line
MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Concentration
1 X
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Sterility
Sterile-filtered
Without Additives
No Glutamine, No HEPES
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Shipping Condition
Room Temperature
Related Products
Description
- Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation
- Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology
- Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
- This Advanced DMEM is manufactured as follows: With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red Without: L-glutamine The complete formulation is available
- Using Advanced DMEM Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
- Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement
- Many cell lines do not require adaptation to this media
- The FBS concentration must be optimized for each cell line to obtain maximum serum reduction
- Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO 2 environment to maintain physiological pH
- Order Info Shipping Condition: Room Temperature
SAFETY INFORMATION
- ShelfLife : 12 Months From Date of Manufacture
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Content And Storage: Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Classification: Animal Origin
Form: Liquid
Serum Level: Reduced Serum
With Additives: High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Green Features: Sustainable packaging
Product Line: Gibco™
Cell Line: MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Concentration: 1 X
Product Type: DMEM (Dulbecco's Modified Eagle Medium)
Sterility: Sterile-filtered
Without Additives: No Glutamine, No HEPES
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Shipping Condition: Room Temperature
Content And Storage:
Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Classification:
Animal Origin
Form:
Liquid
Serum Level:
Reduced Serum
With Additives:
High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Green Features:
Sustainable packaging
Product Line:
Gibco™
Cell Line:
MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Concentration:
1 X
Product Type:
DMEM (Dulbecco's Modified Eagle Medium)
Sterility:
Sterile-filtered
Without Additives:
No Glutamine, No HEPES
Manufacturing Quality:
cGMP-compliant under the ISO 13485 standard
Shipping Condition:
Room Temperature
Content And Storage: Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Classification: Animal Origin
Form: Liquid
Serum Level: Reduced Serum
With Additives: High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Green Features: Sustainable packaging
Product Line: Gibco™
Cell Line: MRC-5, SP2, Vero, WI-38 and Jurkat
Concentration: 1 X
Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterility: Sterile-filtered
Without Additives: No Glutamine, No HEPES
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Shipping Condition: Room Temperature
Content And Storage:
Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Classification:
Animal Origin
Form:
Liquid
Serum Level:
Reduced Serum
With Additives:
High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Green Features:
Sustainable packaging
Product Line:
Gibco™
Cell Line:
MRC-5, SP2, Vero, WI-38 and Jurkat
Concentration:
1 X
Product Type:
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterility:
Sterile-filtered
Without Additives:
No Glutamine, No HEPES
Manufacturing Quality:
cGMP-compliant under the ISO 13485 standard
Shipping Condition:
Room Temperature
Content And Storage: Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Classification: Animal Origin
Form: Liquid
Serum Level: Reduced Serum
With Additives: Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Green Features: Sustainable packaging
Product Line: Gibco™
Cell Line: Sp2, Vero, Raji, Daudi, and Jurkat
Concentration: 1 X
Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility: Sterile-filtered
Without Additives: No Glutamine, No HEPES
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Shipping Condition: __
Content And Storage:
Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
Classification:
Animal Origin
Form:
Liquid
Serum Level:
Reduced Serum
With Additives:
Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Green Features:
Sustainable packaging
Product Line:
Gibco™
Cell Line:
Sp2, Vero, Raji, Daudi, and Jurkat
Concentration:
1 X
Product Type:
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility:
Sterile-filtered
Without Additives:
No Glutamine, No HEPES
Manufacturing Quality:
cGMP-compliant under the ISO 13485 standard
Shipping Condition:
__
Content And Storage: __
Classification: Animal Origin-free
Form: Liquid
Serum Level: Standard Serum Supplementation
With Additives: High Glucose, Glutamine, Phenol Red
Green Features: __
Product Line: __
Cell Line: HeLa, 293, Cos-7, and PC-12
Concentration: 1 X
Product Type: DMEM (Dulbecco's Modified Eagle Medium)
Sterility: Sterile-filtered
Without Additives: No HEPES, No Sodium Pyruvate
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Shipping Condition: __
Content And Storage:
__
Classification:
Animal Origin-free
Form:
Liquid
Serum Level:
Standard Serum Supplementation
With Additives:
High Glucose, Glutamine, Phenol Red
Green Features:
__
Product Line:
__
Cell Line:
HeLa, 293, Cos-7, and PC-12
Concentration:
1 X
Product Type:
DMEM (Dulbecco's Modified Eagle Medium)
Sterility:
Sterile-filtered
Without Additives:
No HEPES, No Sodium Pyruvate
Manufacturing Quality:
cGMP-compliant under the ISO 13485 standard
Shipping Condition:
__