Gibco™ RPMI 1640 Medium, powder

Description

• RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer
• Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas
• We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This RPMI is modified as follows: With: L-glutamine, Phenol Red Without: HEPES, Sodium Bicarbonate The complete formulation is available
• Using RPMI RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
• RPMI 1640 Medium contains biotin, vitamin B 12 , and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
• In addition, the vitamins inositol and choline are present in very high concentrations
• RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO 2 environment to maintain physiological pH
• Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).